Figure 4.

Alteration of N-glycans in SLC39A9 KO Cells

Quantitative analysis of N-glycan species shown in KDELR2, JTB, and SLC39A9 KO cells, and their revertant cells. A schematic of N-glycan biosynthesis is shown in the upper section. Red boxes indicate glycan structure numbers as shown in Data S5. Pink boxes indicate statistically increased glycans, and gray boxes indicate statistically decreased glycans in SLC39A9 KO cells as illustrated in the graphs below. Glycans N3, N4, N5, and N7 may take one of two structures; therefore they are distinguished as N3-1, N3-2, N4-1, N4-2, etc. The graphs show the expression levels of respective N-glycan species compared among the indicated cells (#1–7): mean expression levels ± SD obtained from eight independent experiments. The full raw data set is shown in Data S5. The Bonferroni corrected t test was used for multiple comparisons. *p < 0.025; **p < 0.0083. See also Figures S3 and S4, and Data S5.