Figure 6.

Loss of SLC39A9 Reduces C1GalT1 Proteins

(A) Quantitative real-time PCR of C1GalT1 and MAN2A1 mRNAs in parent cells, SLC39A9 KO cells, wild-type SLC39A9-expressing SLC39A9 KO cells, and H155R mutant-expressing SLC39A9 KO cells. Relative mRNA levels of C1GalT1 and MAN2A1 are expressed as the percentage of the value in parent cells and represent the mean percentages ± SD obtained from three independent experiments.

(B) Western blot analysis for C1GalT1, C1GalT1C1, and MAN2A1 proteins in parent cells, SLC39A9 KO cells, wild-type SLC39A9-expressing SLC39A9 KO cells, and H155R mutant-expressing SLC39A9 KO cells. The amounts of proteins were expressed as a percentage of the intensity of bands in parent cells: mean percentages ± SD. obtained from three independent experiments. The Bonferroni corrected t test was used for multiple comparisons. *p < 0.017.

See also Figure S5.