FIGURE 3.
SpyCEP cleavage of the extended form of CXCL8 (CXCL81–77) results in significantly impaired migration of neutrophils. (A)–(C) show the collated tracks of individual migrating neutrophils (duplicate conditions) pooled from three independent experiments using different donors. Gradients of H575- or H292-treated CXCL81–77 were established in (A) and (B), respectively, whereas (C) shows the lack of neutrophil migration in the absence of chemokine. The total number of tracked cells from all three experiments is shown in the top right-hand corner. (D)–(F) show significant differences in velocity, directionality, and FMI‖ of the data in (A)–(C) following tracking analysis. Error bars represent the SEM. Statistical significances between CXCL81–77 treatments were determined by one-way ANOVA with Tukey posttest. **p < 0.01, ****p < 0.0001.