Table 1.
Methods used in the optimization of an HPLC technique for the separation of deoxycytidine analogs.
| Figure | Column | Mobile Phases | Elution (%, v/v) | Flow Rate mL/min |
|---|---|---|---|---|
| 2 | Agilent C18 (50 × 3 mm, 1.8 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 4.0) C: H2O |
3 min 5% A, 15% B 6 min 20% A, 15% B 6.05 min 30% A, 15% B 9 min 30% A, 15% B 9.05 min 5% A, 15% B 13 min 5% A, 15% B |
1.4 |
| 3 | Luna Phenyl Hexyl (150 × 4.6 mm, 5 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 4.0) C: H2O |
4 min 5% A, 15% B 6 min 20% A, 15% B 6.05 min 30% A, 15% B 9 min 30% A, 15% B 9.05 min 5% A, 15% B |
1.4 |
| 3 (insert) | Luna Phenyl Hexyl (150 × 4.6 mm, 5 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 4.0) C: H2O |
8 min 5% A, 15% B 11 min 20% A, 15% B 11.05 min 30% A, 15% B 16 min 30% A, 15% B 16.05 min 5% A, 15% B |
1.4 |
| 4a | Luna Phenyl Hexyl (150 × 4.6 mm, 5 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 7.0) C: H2O |
8 min 5% A, 15% B 11 min 20% A, 15% B 11.05 min 30% A, 15% B 16 min 30% A, 15% B 16.05 min 5% A, 15% B |
1.4 |
| 4b | Luna Phenyl Hexyl (150 × 4.6 mm, 5 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 7.0) C: H2O |
8 min 3 % A, 15% B 11 min 20% A, 15% B 11.05 min 30% A, 15% B 16 min 30% A, 15% B 16.05 min 3% A, 15% B |
1.0 |
| 4c | Luna Phenyl Hexyl (150 × 4.6 mm, 5 μm) | A: CH3OH B: 50 mM NH4H2PO4 (pH 7.0) C: H2O |
8 min 1% A, 15% B 11 min 20% A, 15% B 11.05 min 30% A, 15% B 16 min 30% A, 15% B 16.05 min 1% A, 15% B |
1.4 |