Table 1.

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) injection mix suggested concentrations.

Aim	Working Concentrations	Components
Indels by NHEJ	Cas9 (50 ng/μL) + gRNA (25 ng/μL)	Cas9 and one gRNA in 30 μL of H2O
Point mutation, small tag insertion or insertion of loxP	Cas9 (50 ng/μL) + gRNA (25 ng/μL) + ssODN (100 ng/μL)	Cas9, one (for point mutation) to four (insertion of loxP) gRNAs, ssODN (120–200 bp) in 30 μL of H2O
deletion of critical exons or rearrangement of larger fragments	Cas9 (50 ng/μL) + gRNA5a (6.5 ng/μL) + gRNA5b (6.5 ng/μL) + gRNA3a (6.5 ng/μL) + gRNA3b (6.5 ng/μL) + ssODN (100 ng/μL)	Cas9, two to four gRNAs, ssODN (120–200 bp) in 30 μL of H2O

NHEJ: non-homologous end joining; gRNA: guide RNA; ssODN: single-stranded oligodeoxynucleotides.