Table 1.
Summarized semiquantitative detection of mRNA coding for proteins involved in Shh release from producing Panc1 cells or proteins involved in Shh signaling in receiving C3H10T1/2 cells. Required proteins for Shh assembly at the cell surface and (proteolytic) release are Dispatched (Disp), Glypican (Gpc) 1–6, and ADAM (A) sheddases 10, 12, and 17. Required proteins for Shh signaling on receiving cells are Patched (Ptc) and Gli family members 1–3.
| Disp1 | Ptc | Shh | Gli1 | Gli2 | Gli3 | Gpc1 | Gp2 | Gp3 | Gpc4 | Gpc5 | Gpc6 | A10 | A12 | A17 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Panc1 | +++ | n.a. | + | - | - | + | +++ | - | - | - | - | + | ++ | - | +++ |
| C3H10T1/2 | + | + | n.a. | - | + | ++ | + | - | - | ++ | - | ++ | n.a. | n.a. | n.a. |