Table 1.
Primary Antibodies Used for IHC and WB
| Antibody | Company | Catalog no. | Dilution | Method |
|---|---|---|---|---|
| VP16-tag | Santa Cruz Biotechnology (Dallas, TX) | sc-7545 | 1:200 | IHC |
| Myc-tag | Cell Signaling Technology Inc. (Danvers, MA) | 2978 | 1:1000 | WB |
| AFP | Proteintech (Rosemont, IL) | 14550-I-AP | 1:2000 | WB |
| DLK1 | Proteintech | 10636-I-AP | 1:1000 | WB |
| GAPDH | EMD Millipore (Billerica, MA) | 5174P | 1:10,000 | WB |
| Yap | Cell Signaling Technology Inc. | 4912S | 1:1000 | WB |
| Phosphorylated Yap | Cell Signaling Technology Inc. | 13619P | 1:1000 | WB |
| TEAD4 | Sigma-Aldrich | HPA056896 | 1:100 | IHC |
| Ki-67 | Thermo Fisher Scientific | MA5-14520 | 1:150 | IHC |
| β-Catenin | BD Biosciences (San Jose, CA) | 610153 | 1:200 | IHC |
AFP, α-fetoprotein; DLK1, Δ-like noncanonical Notch ligand 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IHC, immunohistochemistry; TEAD4, TEA domain transcription factor 4; VP16, virus protein 16; WB, Western blot analysis.