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. 2019 May;189(5):1077–1090. doi: 10.1016/j.ajpath.2019.01.016

Table 2.

Real-Time Quantitative RT-PCR Primer Sequence Information

Gene Forward sequence Reverse sequence
rRNA 5′-CGGCTACCACATCCAAGGAA-3′ 5′-GCTGGAATTACCGCGGCT-3′
Human
 CTGF (CCN2) 5′-CCAATGACAACGCCTCCTG-3′ 5′-GAGCTTTCTGGCTGCACCA-3′
 CYR61 (CCN1) 5′-AGCCTCGCATCCTATACAACC-3′ 5′-GAGTGCCGCCTTGTGAAAGAA-3′
 TEAD1 5′-ATGCCAACCATTCTTACAGTGAC-3′ 5′-ACAGTTCCTTTAAGCCACCTTTC-3′
 TEAD2 5′-GCCTCCGAGAGCTATATGATCG-3′ 5′-TCACTCCGTAGAAGCCACCA-3′
 TEAD3 5′-TGGACCCTCTCAGGACATCAA-3′ 5′-CCAGGGGCTCATAACTGCTG-3′
 TEAD4 5′-GGACACTACTCTTACCGCATCC-3′ 5′-TCAAAGACATAGGCAATGCACA-3′
 YAP (YY1AP1) 5′-TAGCCCTGCGTAGCCAGTTA-3′ 5′-TCATGCTTAGTCCACTGTCTGT-3′
 SURVIVIN (BIRC5) 5′-AGGACCACCGCATCTCTACAT-3′ 5′-AAGTCTGGCTCGTTCTCAGTG-3′
 AXL 5′-TGTAAAACGACGGCCAGT-3′ 5′-CAGGAAACAGCTATGACC-3′
Mouse
 Ctgf (Ccn2) 5′-GGGCCTCTTCTGCGATTTC-3′ 5′-ATCCAGGCAAGTGCATTGGTA-3′
 Cyr61 (Ccn1) 5′-CTGCGCTAAACAACTCAACGA-3′ 5′-GCAGATCCCTTTCAGAGCGG-3′
 Survivin (Birc5) 5′-GCCACGCATCCCAGCTT-3′ 5′-TTTCAAAATACCACTGTCTCCTTCTC-3′
 Axin2 5′-CAGATGAACCTGAAGGATACCAAA-3′ 5′-AGACAACGCTGTTGTTCTCAATG-3′
 Tbx3 5′-CAGGCAGCCTTCAACTGCTT-3′ 5′-GGACACAGATCTTTGAGGTTGGA-3′
 Cyclind1 (Ccnd1) 5′-CGTGGCCTCTAAGATGAAGGA-3′ 5′-CCTCGGGCCGGATAGAGTAG-3′
 Yap 5′-TACTGATGCAGGTACTGCGG-3′ 5′-TCAGGGATCTCAAAGGAGGAC-3′
 Tead1 5′-AAGCTGAAGGTAACAAGCATGG-3′ 5′-GCTGACGTAGGCTCAAACCC-3′
 Tead2 5′-GAAGACGAGAACGCGAAAGC-3′ 5′-GATGAGCTGTGCCGAAGACA-3′
 Tead3 5′-CAACCAGCACAATAGCGTCCA-3′ 5′-CTGAAAGCTCTGCTCGATGTC-3′
 Tead4 5′-CAACCTGGAACATCCCACGAT-3′ 5′-GAAAGCCGAGAACTCCAACAT-3′

CTGF, connective tissue growth factor; Cyr61, cysteine rich angiogenic inducer 61.