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. 2019 May 6;2019:6193186. doi: 10.1155/2019/6193186

Figure 3.

Figure 3

Involvement of LOS in N. meningitidis-induced mRNA expression of inflammasome components. Isolated human neutrophils were challenged by the parental wild-type N. meningitidis FAM20 or its LOS-deficient mutant strain IpxA at MOI 10 or by LPS (50 ng/mL) for 2 h. The mRNA expression of NLRP3 (a), IL1B (b), and CASP1 (c) was measured by RT-qPCR. Relative gene expression was calculated using the 2-∆∆CT method, using GAPDH as the reference gene and the unstimulated cells as the calibrator. Bars represent mean ± SEM (n = 5).