Figure 6: Primary wound isolates result in differential host responses and wound healing.

A) Observed concentration of secreted cytokines (pg/mL) from primary keratinocytes exposed for 8 hours to conditioned media from mature biofilms of A. faecalis (Af), C. striatum (CS), S. aureus 7372 (SA7372), or S. aureus 10757 (SA10757). Each condition was repeated with three biological replicates and three technical replicates of each (n=3 replicates from 3 cell cultures per group). Analysis of variance with post hoc multiple comparison testing was performed between each group (****<0.00001, ***<0.0001, **<0.001, *<0.01, +<0.05). B) Biofilms of each strain listed above were allowed to mature over a period of 72 hours on sterile gauze before being placed into full thickness dorsal mouse wounds (n=4 mice per group). Photographs of the wounds were taken at day 0, 3, 7, 14, 21, and 28. Wound measurements were recorded by two independent observers and are plotted in C) over time. Error bars represent standard error of the mean. D) Representative keratin 14 (K14) immunofluorescence staining of each wound at day 28. E) Gap (μM) between wound edges of each sample (n=4 wounds). A two-sided Wilcoxon-rank analysis was performed between each group (*<0.05).