Table 2.
Primers used in this study for the PCR check and mutagenesis
| Name | Description/Sequence (5´-3´) | Sources |
|---|---|---|
| Primers | ||
| Kt-ra | CGGCCACAGTCGATGAATCC | Datsenko and Wanner [22] |
| rcsA-f | GTGACCCATGTTGTTCCGTTTAG | This study |
| wzc-f | CGCCATATCGAACGCTTATG | This study |
| dgsA-f | CCGTCATCACTCAGGAGGTG | This study |
| glgA-f | CCGGAACTGGATATGTACGATC | This study |
| wzxE-f | GCAGAACGCGCATATGTTCTAC | This study |
| flgA-f | ATTGCGGACAGGTACAATTCAC | This study |
| flhA-f | TGTATCGACATGCGGAGATTG | This study |
| fliQ-f | GTGATAGCCAGCGTGTTGATG | This study |
| lrhA-f | TGCACGAGAGTGGAACAAGG | This study |
| ccmA-f | CTGACGATGGCACAGAATGAG | This study |
| citD-f | CCAGGAGATGCCGATATCCGC | This study |
| grxA-f | GCTTCCCTCTGCAAAGTGAGCC | This study |
| lapA-f | GCGAACGTTAACCATTGCTATC | This study |
| lapA-r | AACGGAACAGGTTTCCGAGCG | This study |
| kdsD-f | CGTGACTACAGCGTGATGTTG | This study |
| lpcA-f | AGCACCTGCCCGTACTTCTCGC | This study |
| arnA-f | CTGACCTTCGGACCACAATG | This study |
| rfbX-f | TGGTTCTGTCTGATATCGCTG | This study |
| wbbI-f | ACAGGTGGAGTCTCTATGTCG | This study |
| ybaY-f | GGCAGAAATGCGTGATGTGTGC | This study |
| cdsA-f | GATGTTCTCTGGCCCGATTTC | This study |
| clsA-f | TCCGTTCTACTCCGCTTCATG | This study |
| pgpA-f | TCACTGTGCCGGAACTGAACC | This study |
| fadR-f | GCAGGAGTGAGGCAAGTCTTG | This study |
| pfk I-f | TGGTTCAGGCACATATGGTG | This study |
| pfk II-f | CATAACGATGGCAGGAACTGTC | This study |
| sthA-f | CCCATCACGATGTCTGAATCC | This study |
| nuoA-f | TATCCTGGAGTCGTCAAGGATC | This study |
| nuoB-f | TCGCATGGTCAACCTCTATCC | This study |
| nuoE-f | CAGAGTCTGCGCATTCTTGAG | This study |
| nuoF-f | GCGTGGTCTGTCATATCAACG | This study |
| nuoG-f | GTGCAGTGGAGCCGTTACAG | This study |
| nuoN-f | GGCAGCTTCCAGGTTGTACC | This study |
| pgi-f | CCTGTAGCCGATGATGAACG | This study |
| narG-f | CCACGCTGTTTCAGAGCGTTAC | This study |
| H1P4-fliQ | TGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGATTCCGGGGATCCGTCGACC | This study |
| H2P1-fliQ | GTTCGCTTGTCACCTGCAACATAGTACGGCTACCCGATGATATACGGCAGTGTAGGCTGGAGCTGCTTCG | This study |
| Plasmids | ||
| pBAD-NfsB | pBAD vector with AmpR harbouring the nitroreductase NfsB | Valle [18] |
| pCP20 | AmpR and CmR plasmid; thermal induction of FLP synthesis | Datsenko and Wanner [22] |
| pKD46 | AmpR plasmid contains red system for homologous recombination | Datsenko and Wanner [22] |
| pKD13 | KanR plasmid; was used for generating the cassette with kanamycine resistance gene | Datsenko and Wanner [22] |
| Constructed strain | ||
| lapAfliQ::kan | KanR mutant | This study |
ApR , KanRand CmR indicate ampicillin, kanamycin and chloramphenicol resistance
aAll mutant strains were checked by the kt-r reverse primer of internal kanamycin resistance cassette [22]