Table 1.
The keystone taxa in the bacterial and fungal networks under non-amended and biochar-amended treatments
| Network | OTU ID | Module | Degree | Cluster coefficient | Abundance (%)a | Phylum/class | Affiliation | Z b | P b | Diversity | Carbohydrate | qCO2 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Field | SIP incubation | ||||||||||||
| Bacteria | |||||||||||||
| Non-amended | OTU2131 | Module I | 8 | 0.22 | 0.25 | 1.15 | Alphaproteobacteria | Sphingomonas | 2.67 | 0.22 | − 0.357 | 0.627* | 0.602* |
| Biochar-amended | OTU717 | Module I | 8 | 0.23 | 0.20 | 12.39 | Actinobacteria | Arthrobacter | 3.12 | 0 | 0.798*** | − 0.879*** | − 0.756** |
| OTU316 | Module II | 9 | 0.29 | 0.09 | 0.18 | Gemmatimonadetes | Gemmatimonadaceae | 2.95 | 0.19 | 0.617* | − 0.841*** | − 0.789*** | |
| Fungi | |||||||||||||
| Non-amended | OTU148 | Module II | 6 | 0.35 | 0.05 | 5.44 | Eurotiomycetes | Aspergillus | 2.53 | 0 | − 0.479 | 0.763** | 0.762** |
| Biochar-amended | OTU430 | Module II | 7 | 0.57 | 0.26 | 0.09 | Sordariomycetes | Chaetomium | 2.57 | 0 | 0.832*** | − 0.722** | − 0.654** |
| OTU516 | Module II | 7 | 0.62 | 0.14 | 7.14 | Eurotiomycetes | Penicillium | 2.57 | 0 | 0.609* | − 0.722** | − 0.642** | |
qCO2, soil metabolic quotient. *P < 0.05; **P < 0.01; ***P < 0.001.
aAbundance of keystone species in the field experiment and stable isotope probing (SIP) microcosms
bThe topological role of each node is determined based on two properties: the within-module connectivity Z and the among-module connectivity P