Figure 3.

Classical monocyte proportions increased in the lungs of cAIMP‐treated humanized mice. (a–e) These panels present the mouse lung cell flow cytometry gating strategy. Gates were set based on singlets (SSC‐A/SSC‐H), CD45⁺ and subsequently on FSC/SSC to divide cells into a lymphocyte and a mono‐granulocyte compartment (a). Within this gate, cells were divided into CD33neg, CD33dim, CD33bright populations (b). These populations were further characterized based on CD14 and CD16 expression (c–e). (f,g) Proportions of CD33bright cells in Low‐Hu mice (b) are shown for each mouse in (f). The proportions of CD33dimCD16⁺ CD14neg cells in these same animals (d) are shown in (g). Values are presented as percentages of total CD45⁺ cells. Each symbol represents one mouse. One‐way ANOVA Kruskal–Wallis with Dunn's multiple comparisons results that met the alpha of 0·05 are noted with P‐values above the data within the figure.