Table 1.
Cocktails of antibodies used for double and triple labeling immunohistochemistry
| Primary antibody/stain | Dilution | Host | Company | Secondary antibody |
|---|---|---|---|---|
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
| ChAT | 1:100 | goat | Millipore | donkey anti-goat Cy3 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
| DARPP-32 | 1:200 | guinea pig | Syn. Systems | donkey anti-guinea pig Cy3 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
| Ucn-1 | 1:100 | goat | Santa Cruz | donkey anti-goat Cy3 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
| TH | 1:50 | goat | Santa Cruz | donkey anti-goat Cy3 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | biotin-donkey anti-rabbit + SA-HRP |
| ThS | 1% | - | Sigma | - |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
| Abeta (4G8) | 1:2000 | mouse | Biolegend | donkey anti-mouse Cy2 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
| Iba1 | 1:200 | guinea pig | Syn. Systems | donkey anti-mouse Cy5 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
| GFAP (G-A-5) | 1:1000 | mouse | Sigma | donkey anti-mouse Cy5 |
| HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
| ThS | 1% | - | Sigma | - |
| GFAP (G-A-5) | 1:1000 | mouse | Sigma | donkey anti-mouse Cy5 |
Secondary antibodies were all from Dianova and used at a dilution of 1:400
HTT huntingtin, ChAT choline acetyltransferase, DARPP-32 dopamine and cAMP regulated phosphoprotein 32 kDa, Ucn-1 Urocortin-1, TH tyrosine hydroxylase, SA-HRP horseradish peroxidase-conjugated streptavidin, Iba1 ionized calcium-binding adapter molecule 1, ThS ThioflavinS, GFAP glial fibrillary acidic protein