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. 2019 Apr 30;132(9):jcs224311. doi: 10.1242/jcs.224311

Fig. 2.

Fig. 2.

XMAP215 KD disrupts MT organization in the growth cone. (A) Representative SIM images of growth cone MTs considered as ‘splayed’ and ‘looped’. XMAP215 KD increases the MT looped morphology compared to controls. Scale bar: 3 µm. (B) Representative growth cone images of control and XMAP215 KD neural explants immunostained for tyrosinated tubulin (Tyr tub) and detyrosinated tubulin (Detyr tub) to label dynamic versus stable MTs, respectively. XMAP215 KD increases the dynamic:stable MT ratio. Scale bar: 5 µm. a.u., arbitrary units. (C) Representative SIM image of growth cones stained for actin and tubulin. The labeled lines letter indicate how the MT measurements were performed for the corresponding plots in D–F. Scale bar: 8 µm. (D–F) Quantification of the different MT parameters. XMAP215 KD increases the MT–MT distance in growth cone neck (D), the MT–MT distance in the growth cone's widest region (E), as well as the growth cone length (F). **P<0.01; ***P<0.001; ****P<0.0001; ns, not significant, from a Student's t-test comparing XMAP215 KD to control. Data presented as mean±s.e.m.