Fig. 7.
Tubacin treatment rescues organoid branching in paxillinfl/flcre organoids. (A) Phase images of day 6 organoids treated with 2 μM tubacin for 4 days. Scale bar: 40 μm. (B) Quantification of branching at day 2, day 4 and day 6, n=3 (total of 18-82 organoids per treatment). One-way ANOVA with Tukey's multiple comparisons test was performed for statistical analysis. (C) Whole-mount staining of organoids showing paxillin and acetylated-tubulin expression patterns in the tubacin-treated organoids and DMSO-treated controls. Scale bar: 20 μm. (D) Phase images of 0.1 μM paclitaxel-, 200 ng/ml nocodazole- and DMSO-treated paxillin+/+cre and paxillinfl/flcre organoids. Scale bar: 20 μm. (E) Quantification of branching in drug-treated organoids, n=3. Student's t-test was performed. Data are mean±s.e.m. n.s., not significant; **P<0.01, ***P<0.001.