Table 1.

Current Methods for Preparing and Analyzing Peptide Arrays^a

		Merrifield synthesis	SPOT		SAMDI	photolithography		particle-based
technical components	peptide synthesis	SPPS	in situ		SPPS	in situ		in situ
	solid support	glass	nitrocellulose or PVDF membranes		gold on glass/metal	functionalized glass or silicon		functionalized glass
	surface chemistry	several methods available, e.g., polymer surface coating	amino derivitazation, typically with B-alanine or PEG		self-assembled mono-layersmaleimide and EG3 terminated	amino-derivatized polymer		amino-derivatized polymer (PEGMA)
	inert layer	surface blocker with BSA or other blocking agents	membranes blocked with BSA or other blocking agents		self-assembled monolayers tri(ethylene) glycol	surfaces blocked with BSA or other blocking agents		surfaces blocked with BSA or other proteins
	immobilization	many, e.g., cysteine-terminated peptide on glyoxylyl, bromomethylketone or disulfide surfaces	coupling of carboxyl group to aminofunctionalized surface		many, cysteine terminated peptides on maleimide surfaces	coupling of carboxyl group to amino functionalized surface		coupling of carboxyl group to amino functionalized surface
	patterning method	liquid handler	liquid handler	inkjet printer	liquid handler	masks	mircomirrors/ digital patterning	laser printer
	maximum feature density	318 spots/cm2	5 spots/cm2	4000 spots/cm2	16 spots/cm2	694 000 spots/cm2	1 000 000 spots/cm2	40 000 spots/cm2
	minimum feature size	250 μm	2–3 mm	90 μm	1.25 mm	8 μm	10 μm × 10 μm	50 μm
detection methods	radioactivity	×	×		×	×		×
	chemilluminescence	×	×		×	×		×
	colorimetry	×	×		×	×		×
	fluorescence	×	×		×	×		×
	mass spectrometry				×
	SPR	×			×
	commercialization	PEPSCAN, New England Peptide, Biosynthesis	JPT, Intavis, Arrayit, MultiSynTech, Kinexus, Genscript	Arrayjet	SAMDI Tech	–	LC Sciences, Schafer-N	PEPperPRINT

^aReferences can be found in the text.