Figure 6.

The silencing of TLR9 inhibits migration, whereas Helicobacter pylori (H. pylori) DNA promotes cell migration in MKN45 cells. MKN45 cells were transfected with the scrambled miRNA or indicated miRNA and cocultured with DNA, respectively. Cells that migrated into the lower wall were stained and counted under a microscope. For quantitative assessment, the number of migrating cells stained with crystal violet was lysated and detected at 570 nm on a plate reader. **P < 0.01, compared with the blank group; ^##P < 0.01, compared with the NC + DNA group