Figure 1.

A Single-Cell Proteomic Atlas of Breast Cancer Ecosystems

(A) Experimental approach.

(B) t-SNE plots of EpCAM, CD45, CD31, and FAP expression in 58,000 cells from all samples using a 0 to 1 normalization.

(C) t-SNE as in (B), colored by cell type.

(D and E) Frequencies of live epithelial cells, immune cells, endothelial cells, and fibroblasts for (D) mammoplasty (M), juxta-tumoral (JT), and tumor (T) samples and (E) tumor subtypes.

Wilcoxon rank-sum test was used for statistical analysis. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. See also Figure S1.