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. 2019 May 13;14:3503–3516. doi: 10.2147/IJN.S202379

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© 2019 Li et al.

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Antigen delivery efficiency of arginine-containing cationic liposomes in BMDCs. Alexa Fluor 555-conjugated OVA (fc: 2.5 μg/mL) was freely dispersed or loaded on cationic liposomes (fc: 25 μM), and incubated with BMDCs for 2 hrs. The extracellular OVA-liposomes were washed off, and the intracellular OVA was measured by flow cytometry. (A) Cells were first gated by FSC-A vs SSC-A, and then gated by SSC-A vs SSC-H. (B) Representative histograms and (C) mean fluorescent intensities of intracellular OVA. Data show the mean + SD of two independent experiments with two replicates, respectively.

Abbreviations: BMDCs, bone marrow-derived dendritic cells; OVA, ovalbumin; FSC, forward scatter; SSC, side scatter; A, area; H, height.