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Suppression of autophagy by LPS is TLR4-dependent. (a) The genotyping of tlr4−/− mice by RT-PCR. (b) Autophagic flux analysis in tlr4−/− microglia after treatment with LPS (1 μg/mL) for 24 h. BafA1 (20 nM) was added 2 h before sampling. (c) Analysis of the mRNA levels of Atg genes in tlr4−/− microglia by qRT-PCR 24 h after LPS (1 μg/mL) treatment (n = 4).
