Table 1.
ATG Mutant alleles recovered as lon2-2 suppressors in this work.
| Gene (accession number) | Protein function | Allele | Alias1 | Nucleotide change | Protein or transcript change |
|---|---|---|---|---|---|
| ATG2 (At3g19190) | Phagophore expansion | atg2-6 | L60 | g6271a | Intron 6 splice-acceptor site |
| atg2-7 | L78, L79 | g795a | W90Stop | ||
| ATG3 (At5g61500) | ATG8 lipidation (E2-like) |
atg3-2 | L13, L14, L15, L16 | g1613a | Intron 5 splice-acceptor site |
| ATG5 (At5g17290) | ATG8 lipidation (E3-like) |
atg5-5 | L40, L41 | g1387a | Intron 4 splice-acceptor site |
| atg5-6 | L43, L44, L45 | g2615a | E323K | ||
| ATG7 (At5g45900) | ATG8 lipidation (E1-like) |
atg7-62 | L61 | g1648a | W344Stop |
| atg7-10 | L21, L23, L24, L27 | g140a | Intron 1 splice-donor site | ||
| atg7-11 | L160 | g552a | G91E | ||
| atg7-12 | L37, L46 | g715a | Intron 2 splice-acceptor site | ||
| atg7-13 | L17, L18 | g721a | W119Stop | ||
| atg7-14 | L123 | g940a | W192Stop | ||
| atg7-15 | L39 | g1454a | W307Stop | ||
| atg7-16 | L66 | g2269a | Intron 7 splice-acceptor site | ||
| atg7-17 | L20, L25 | g2394a | G535D | ||
| atg7-19 | L71 | c2562t | T559I | ||
| atg7-21 | L92, L93, L96, L97 | c2848t | Q629Stop | ||
| ATG16 (At5g50230) | ATG8 lipidation (membrane tethering) | atg16-1 | L65 | g662a | W47Stop |
| atg16-2 | L68 | g650a; g760a |
Intron 2 splice-acceptor site; R80K | ||
| atg16-3 | L53 | c810t | Q97Stop | ||
| ATG18a (At3g62770) | Phagophore expansion | atg18a-3 | L1 | c463t | Q155Stop |
| atg18a-4 | L154 | g1921a | W364Stop |
1In all but one case, multiple isolates of allelic mutations were from the same M2 pool, indicating that the isolates were likely siblings. The exception was atg7-12, which was isolated from 2 independent pools.
2atg7-6 was independently isolated in a pilot lon2-2 suppressor screen [48].