Figure 7.

Nuclear translocation of the PDIA3-STAT3 protein complex modulated MCOLN3 expression. (a) HFE145 cells were analyzed for protein levels as indicated by immunoprecipitation (IP) or western blots. Ten percent of the lysate used for the IP was loaded as input (n = 3). (b,c) HFE145 cells were incubated with 1,25D3 (200 nM) for 12 h. (b) Protein levels of PDIA3 and STAT3 in the cytosolic and nuclear extracts were determined and quantified. ACTB and LMNB1 were used as loading controls for the cytosolic and nuclear fractions, respectively. (c) STAT3 binding to the MCOLN3 promoter was analyzed by ChIP-PCR. Normal IgG was used as an internal control. (d) HFE145 cells transfected with control or STAT3-specific siRNA were incubated with 1,25D3 (200 nM, 48 h). Cell lysates were examined for STAT3 and MCOLN3 protein levels. MCOLN3 proteins were quantified. All the quantitative data are presented as means ± S.E.M. from 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.