Table 7.
Inhibition of cytosolic AMP hydrolysis by selected CD73 inhibitors investigated in a CD73-knockout melanoma cell line.
| Compound (concentration) | Inhibition of AMP hydrolysis ± SEM (%)a |
|---|---|
| Levamisole (1 mM) and NaF (5 mM) | 70 ± 1 |
| 4l (100 µM) | 4 ± 13 |
| 7f (100 µM) | −3 ± 6 |
| 9e (100 µM) | −5 ± 5 |
| 9h (100 µM) | −2 ± 3 |
a
Determined in a malachite green assay using a cytosolic extract of MaMel.65-CD73ko cells (for details see Experimental Section). Data are from three independent experiments performed in duplicate.