Table 2.
Oligonucleotides used in the study.
| Primer | Sequence (5ʹ → 3ʹ) | Locus | Ta [°C] | PCR [bp] | Ref. |
|---|---|---|---|---|---|
| Pap1 Pap2 |
GACGGCTGTACTGCAGGGTGTGGCG ATATCCTTTCTGCAGGGATGCAATA |
papC | 60 | 328 | Adamus-Bialek et al., 2009 |
| Sfa1 Sfa2 |
CTCCGGAGAACTGGGTGCATCTTAC CGGAGGAGTAATTACAAACCTGGCA |
sfaD/sfaE | 60 | 410 | |
| Cnf1a Cnf2a |
AAGATGGAGTTTCCTATGCAGGAG CATTCAGAGTCCTGCCCTCATTATT |
cnf1 | 60 | 498 | |
| Usp1mod Uspe2mod |
TTCTGGGGAACTGACATTCACGG CCTCAGGGACATAGGGGGAA |
usp | 60 | 657 | |
| FimGH1 FimGH2 |
GCAATGTTGGCGTTCGCAAGTGC CGTAAATATTCCACACAAACTGG |
fimG/H | 60 | 1001 | |
| Hly1mod Hly2mod |
AACAACGATAAGCACTGTTCTGGCT ACCATATAAGCGGTCATTCCCATCA |
hlyA | 60 | 1177 |