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. 2019 Apr 16;10(1):292–314. doi: 10.1080/21505594.2019.1598172

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Interactions of Mopex13, Mopex14, Mopex14/17 and PTS receptors. The interactions between Mopex13, Mopex14 and Mopex14/17 (a), and the interactions of Mopex13, Mopex14 and Mopex14/17 with Mopex5 and Mopex7 (b) were analyzed by the yeast two-hybrid assay. The proteins were fused to the GAL4 DNA-binding domain (GAL4-BD) and the GAL4-activation domain (GAL4-AD), respectively. The indicated plasmid combinations were cotransformed into the Y2HGold yeast strain (Clontech). The transformants were tested for blue coloration on leucine/tryptophan double dropout plates presenting X-a-Gal and for viability on leucine/tryptophan/adenine/histidine quadruple dropout plates.