Figure 9.

Mopex13 and Mopex14 are involved in lipid metabolism. (a) Lipid utilization by ∆mopex13 and ∆mopex14 and the wild-type strain Guy-11. The strains were cultured on minimal medium with glucose (1%), Tween 80 (0.5%), olive oil (0.5%) or sodium acetate (50 mM) as the sole carbon source for 5 days. (b) Relative transcription of MoPEX13 and MoPEX14 in the wild type cultured on minimal medium with glucose (1%) or with glucose (0.5%) together with Triolein (0.5%), Tween 80 (0.5%), olive oil (0.5%) or sodium acetate (50 mM). (c) Deletion of MoPEX13 or MoPEX14 delayed lipid mobilization and degradation during appressorium development. Conidia of the wild-type strain Guy-11 and mutants were incubated on a hydrophobic membrane and allowed to form appressoria. Samples at different time points were stained with Nile Red and examined microscopically. Bar = 5 μm. (d) Inoculation of barley leaves with conidial suspensions (1 × 10⁵ conidia/ml) supplemented with glucose (2.5%). The presence of glucose partially improved the ability of the mutants to cause disease.