Figure 2.

Establishment of the HEK293-PGRN-T2A-Luciferase-KI cell line. (a) The HEK293-PGRN-T2A-Luciferase-KI cell line with stable expression of eGFP and undetectable expression of mCherry. (b) Genotyping of the positive clones contained a lower band corresponding to the WT and an upper band corresponding to the targeted integration (TI). (c) Sequencing results of the 5ʹ and 3ʹ terminal sequences from the upper band of clone 4 in panel B. (d) Sequencing results of the up homologous arm from the upper band of clone 4 in panel B. (e) Sequencing results of the down homologous arm sequences from the upper band of clone 4 in panel B. (f) Sequencing results from the lower band of clone 4 in panel B. The lower band contained a 5 bp deletion.\