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. 2019 Apr 26;10(1):98–107. doi: 10.1080/21655979.2019.1607126

Figure 4.

Figure 4.

The effect of small molecule chemical drugs on PGRN transcription regulation. (a) Different doses of PMA were added to HEK293-PGRN-T2A-Luciferase-KI cell line. The luciferase activity was detected 48 h post-treatment with PMA. (b) The endogenous PGRN mRNA level was assayed in the HEK293 and HEK293-PGRN-T2A-Luciferase-KI cell lines in the presence of PMA by real-time PCR. The effect of PMA (2.5 ng/ml) on the endogenous PGRN mRNA level (c) and the PGRN protein level from supernatant (d) were analyzed in the HEK293 and the HEK293-PGRN-T2A-Luciferase-KI cell lines. (e) A library of 28 inflammation and lipid metabolism related small molecules were screened in HEK293-PGRN-T2A-Luciferase-KI cell line. (f) Different doses (2.5, 5, 10, 20 µM) of S2349 were added to HEK293-PGRN-T2A-Luciferase-KI cell line. The luciferase activity was detected 48 h post-treatment with S2349. (g) The endogenous PGRN mRNA level was assayed in the HEK293 treated with S2349 (10 µM) by real-time PCR. NC, negative control; ns, no significant. Statistically significant differences were indicated: *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test.