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. 2019 Apr 17;10(1):87–97. doi: 10.1080/21655979.2019.1604037

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Schematic of the gene constructions in pBI121 expression vector (a) and the cDNA sequence of the designed synthetic (AP)₂₀ gene (b). When the gene constructs encoding the (AP)₂₀-tagged AAT are expressed in BY-2 cells, the clustered non-contiguous Pro (P) residues in the (AP)₂₀ module are expected to hydroxylate to be Hyp, and subsequently O-glycosylated with arabinogalactan polysaccharides [22,30]. (AP)₂₀: twenty tandem repeats of ‘Ala-Pro’ motif; SS^tob: tobacco extensin signal sequence; CaMV35S: 35S cauliflower mosaic virus promoter; 3ʹNOS: nopaline synthase terminator; His: 6 × His tag.