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. 2019 Apr 17;10(1):87–97. doi: 10.1080/21655979.2019.1604037

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Expression of recombinant AAT products in tobacco BY-2 cell culture. (a) Transgenic BY-2 cell colonies appeared in a selection medium (MS medium with 100 mg/L kanamycin) 3 weeks after co-culture of wild-type BY-2 cell with Agrobacterium; (b) PCR detection of the target gene integration into the genome of transgenic BY-2 cell lines. L: DNA ladder, Lane 1, 2, 3, 4: PCR amplicons amplified from the genomic DNA extracted from the BY-2 cells expressing AAT-(AP)₂₀, AAT control, wild type line, and (AP)₂₀-AAT, respectively; (c) Secreted yields of AAT-(AP)₂₀, (AP)₂₀-AAT and AAT control in the transgenic BY-2 cell cultures. Five top-expression BY-2 colonies for each gene construct (# 1 to 5 on X-axis) selected by dot blotting were grown in SH medium for 10 days before the assay; (d) Intracellular AAT contents of the BY-2 cells. Three top-secretion BY-2 colonies for each construct identified in Panel (c) were assayed. Statistical significance is indicated by *(p < 0.05) and ***(p < 0.01).