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. 2019 Apr 17;10(1):87–97. doi: 10.1080/21655979.2019.1604037

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Biological activities of the recombinant AAT products secreted into BY-2 cell culture media. The medium samples harvested after 10-day of BY-2 cell culture as well as the purified AAT-(AP)₂₀ fusion protein (pAAT-(AP)₂₀) were assayed. The percentage of the active AAT accounting for the total AAT products in the samples was then calculated as: active AAT content/total AAT concentration×100%. The error bars represent the standard deviation of three top-expression cell lines for each gene construct (for the medium samples) or three parallel samples (for the pAAT-(AP)₂₀ protein).