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. 2019 Mar 5;10(1):1–12. doi: 10.1080/21655979.2019.1586056

Figure 6.

Figure 6.

MiR-485-5p targets 3′-UTR of FLOT2 mRNA to inhibit its translation. (a), bioinformatics analysis revealed a miR-485-5p-binding site in the 3ʹUTR of FLOT2 mRNA. (b), Western blotting of FLOT2 protein in NCI-H446 cells transfected with miR-485-5p mimics, miR-485-5p inhibitor or their corresponding controls (miR-NC, inhibitor-NC), respectively; (c), comparison of FLOT2 protein levels among NCI-H1688 cells transfected with miR-485-5p mimics, miR-485-5p inhibitor or their corresponding controls; (d), bioinformatics analysis revealed a miR-485-5p-binding site in the 3ʹUTR of FLOT1 mRNA and Western blotting of FLOT1 protein in NCI-H446 cells and NCI-H1688 transfected with miR-485-5p mimics and miR-NC. FLOT2 protein levels were determined by Western blot assay and normalized to GAPDH. The data represent the mean ± SEM from three independent experiments (n = 3, *p < 0.05).