Figure 5.

Targeting MIAT protects against I/R-induced cell apoptosis via inhibition of p65 nucleus translocation and PUMA signal expression.

a, Representative pictures of sections stained with TUNEL (×400). TUNEL-positive nuclei are shown in green. Blue fluorescence indicated total cardiomyocyte nuclei. Quantitative analysis (percentage of apoptotic cells versus total) is shown in histogram. b, NF-KB activity was detected by EMSA assay; c, Western blots of p65 nucleus translocation, cleaved caspase-3,PUMA and Bax in I/R tissues in the presence or absence of Lv-MIAT shRNA. Vs Lv-NC shNA, **p < 0.01; Vs I/R, *p < 0.05