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. 2019 May 6;10(1):133–141. doi: 10.1080/21655979.2019.1607709

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of S100A9 silencing on cell proliferation. (a) S100A9 mRNA expression in 6 OS cell lines. Expression of S100A9 mRNA was normalized to that of b-actin mRNA. All six OS cell lines were positive for the expression of S100A9 mRNA. The median value was 2.6. MG63 and U2OS cells showed moderate expression of S100A9 mRNA. (b) RNAi induced inhibition of S100A9 mRNA. Two siRNAs against S100A9 were designed (siRNA1 and siRNA2). siRNA (100 pmol) was transfected into cancer cells with the Nucleofector reagent. S100A9 mRNA expression was evaluated in MG63 (a) and U2OS (b) cells at 24 h (a) and/or 48 h (a and b) after transfection. Both siRNAs showed efficient inhibition of transcript expression. Inhibition by siRNA2 was stronger than that by siRNA1. (c) Effect of inhibition of S100A9 mRNA on the proliferation of MG63 (a) and U2OS (b) cells. Cells were seeded after transfection of siRNA and evaluated by MTT assay on days 1, 2, and 4 (a) or on days 1, 3, and 5 (b) after transfection. The proliferation of MG63 and U2OS cells was inhibited. The degree of inhibition of cell proliferation was correlated with that of S100A9 mRNA in MG63 cells.