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. Author manuscript; available in PMC: 2019 May 20.
Published in final edited form as: Clin Immunol. 2015 Nov 26;162:100–106. doi: 10.1016/j.clim.2015.11.010

Figure 2.

Figure 2

MiR-21 deficiency down-regulates components of the CD28:CD80/86 co-stimulatory axis. cGVH reactions were induced in wild-type (WT) and miR-21-deficient (miR-21−/−) hosts as described in Figure 1. Six weeks post-induction host splenocytes were analyzed by flow cytometry for components of the CD28:CD80/86 co-stimulatory pathway. Bar graphs show mean expression of (A) CD4+ CD28+ (B) CD19+ CD80+ and (C) CD19+ CD86+ in miR-21−/− recipients relative to WT controls. Scatter plots shown are representative of six independent experiments. Live/dead cell gates were drawn on the basis of forward-scatter/side scatter. Numbers in the panels represent cell counts of the indicated population in the lymphocyte gate. *p < 0.05 and ***p < 0.005 by Mann–Whitney unpaired Student’s t test with n = 9 independent experiments. Error bars represent the standard error of the mean.