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. Author manuscript; available in PMC: 2019 May 20.
Published in final edited form as: Clin Immunol. 2015 Nov 26;162:100–106. doi: 10.1016/j.clim.2015.11.010

Figure 4.

Figure 4

miR-21 regulates Treg and Th17 differentiation. cGVH reactions were induced in wild-type (WT) and miR-21-deficient (miR-21−/−) hosts as described in Figure 1. Six weeks post-induction host splenocytes were subjected to flow cytometry analysis for expression of (A) CD4+ CD25+ FoxP3+ T-regulatory (Treg) and (B) CD4+ IL-17+ T-helper 17 (Th17) cell markers. Scatter plots shown are representative of six independent experiments. Live/dead cell gates were drawn on the basis of forward-scatter/side scatter. Numbers in the panels represent (C) CD25+ FoxP3+ cell counts in the lymphocyte → CD4+ cell gate and (D) CD4+ IL-17+ cell counts in the lymphocyte gate. ***p < 0.005 by Mann–Whitney unpaired Student’s t test with n = 9 independent experiments. Error bars represent the standard error of the mean.