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Silencing of GPT induces Baf-sensitive degradation of long-lived proteins. LNCaP cells were transfected with a nontargeting control siRNA (siCtrl) or two different GPT-targeting siRNAs. Subsequently, the cells were treated as indicated for 6 h, before measurement of LLPD (mean ± S.E. (error bars), n = 3). Red dots represent individual data points. Statistical significance was evaluated using repeated measures two-way ANOVA. **, p < 0.01; ***, p < 0.001. N.S., not significant.
