Figure 4.

TM induces Baf-sensitive degradation of long-lived proteins in HeLa and RPE-1 cells. HeLaT (A) or RPE-1 (B) cells were treated for 24 h, as indicated, with Baf included the last 4 h only. LLPD was measured at 20–24 h (mean ± S.E. (error bars), n ≥ 3 for HeLaT, n = 3 for RPE-1). Red dots represent individual data points. Statistical significance was evaluated using regular two-way ANOVA in A (HeLaT) and repeated measures two-way ANOVA in B (RPE-1). *, p < 0.05; **, p < 0.01; ***, p < 0.001. N.S., not significant.