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. 2019 Mar 29;294(20):8197–8217. doi: 10.1074/jbc.RA118.002829

Figure 7.

Figure 7.

ER stress–induced autophagy requires ATG13 and GABARAPs, but not WIPI1 or LC3s. A and B, LNCaP cells were siRNA-transfected, followed by treatment for 24 h, as indicated. LLPD was measured at 18–24 h (mean ± S.E. (error bars), n = 3). C, LNCaP cells were siRNA-transfected, followed by treatment for 24 h, as indicated, with Baf included the last 3 h only. LDH sequestration was determined at 21–24 h (mean ± S.E., n ≥ 3). D, LNCaP cells were transfected with siCtrl (0.025 nm) or siATG13 at the specified concentrations and treated for 24 h, as indicated. In parallel, we determined LLPD at 18–24 h (top; mean ± S.D. of three replicates), p-ATG13 and ATG13 protein levels by immunoblotting (middle), and ATG13 mRNA levels by real-time RT-PCR (bottom; mean ± S.D. of three replicates). One representative of two independent experiments is shown. E, LNCaP cells were siRNA-transfected, followed by treatment for 24 h, as indicated. LLPD was measured at 18–24 h (mean ± S.E., n = 3). Red dots represent individual data points. Statistical significance was evaluated using repeated measures two-way ANOVA (A, B, and E) or regular one-way ANOVA (C). **, p < 0.01; ***, p < 0.001. N.S., not significant.