Figure 4.

Inhibitory effects of TFMI-2 variants and SGMI-2 in 2-fold diluted NHS assessed by pathway-selective ELISA tests. All inhibitors inhibited the lectin pathway (black pentagons) in a concentration-dependent manner, whereas the activities of the classical (open gray pentagons) and the alternative (solid gray pentagons) pathways were unaffected. The IC₅₀ values are between 430 nm and 15.4 μm (Table 2). In terms of IC₅₀ value ratios, all TFMI-2 variants are 13.4–35.8-fold more potent LP inhibitors than SGMI-2. Data points represent the average of four (in the case of the LP) or two (in the case of the CP and the AP) experiments. The error bars represent the S.E.