Figure 4.

Mfn1^F202L requires WT Mfn2 to function in a heterotypic complex. A, representative images of a clonal population of Mfn1^−/− Mfn2^−/− + Mfn1^F202L-FLAG stably expressing the indicated Mfn-mNeon. Mitochondria were labeled with MitoTracker Red CMXRos and visualized by fluorescence microscopy. Images represent maximum intensity projections. Scale bars are 5 μm. B, quantification of the mitochondrial morphology of cell lines described in A. Error bars indicate mean + S.D. from three blinded experiments (n > 100 cells per cell line per experiment).