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A and B, stimulation of HEK293-Blue reporter cells expressing hTLR4 (A) or mTLR4 (B) with purified LPS of strain B213 or mutant derivatives. After incubation of HEK293-Blue cells with the indicated LPS variants for 17 h, alkaline phosphatase activity was determined. Grafts show the log response ratio from three independent experiments with average and standard deviation (error bars). Statistically significant differences were analyzed with GraphPad Prism 6. *, p < 0.05.
