. 2019 Apr 2;294(20):8238–8258. doi: 10.1074/jbc.RA118.004415

Table 3.

Indomethacin-induced alteration in mitochondrial functional integrity in rat gastric mucosa and effect of Mdivi-1 in the pathology

Rats were pretreated with Mdivi-1 at 2 mg/kg body weight followed by oral gavaging with indomethacin for 4 h. Control rats were treated with vehicle. Mitochondria were isolated for analyzing β-oxidation of fatty acids, mitochondrial dehydrogenase activity, ETC complex-I, RCR, ATP content, and ΔΨm as described under “Experimental procedures.” All experiments were done in triplicate (with n = 6–8 in each group). Data are presented as means ± S.D. **, p < 0.01; ***, p < 0.001 versus control; ^#, p < 0.05; ^##, p < 0.01 versus indomethacin; NS, not significant.

	Fatty acid oxidation	Mitochondrial dehydrogenase activity (MTT reduction, OD_{570 nm})	ETC complex I activity	Respiratory control ratio (state 3/state 4)	ATP content	ΔΨm (fluorescence 590/530 nm)
	pmol CO₂/g/h		nmol/min/mg		relative luciferase units
Control	1675.2 ± 126.9	0.87 ± 0.02	55.15 ± 4.0	5.29 ± 0.93	6355.00 ± 241.89	7.10 ± 0.35
Indomethacin	698.7 ± 137.3**	0.48 ± 0.05**	14.06 ± 1.4***	1.78 ± 0.14**	2835.33 ± 465.54***	3.10 ± 0.1**
Mdivi-1 + indomethacin	1270.9 ± 121.3^##	0.76 ± 0.04^##	38.28 ± 1.1^##	3.060 ± 0.43^#	4582.67 ± 285.55^#	6.02 ± 0.52^#
Mdivi-1	1483.3 ± 107.4^NS	0.86 ± 0.03^NS	51.12 ± 5.9^NS	5.18 ± 0.80^NS	5727.32 ± 533.59^NS	6.72 ± 0.37^NS