Figure 2.

PP1A dephosphorylates NACA in vitro and in vivo. A, recombinant NACA (arrow) was treated with the indicated recombinant enzymes for 90 min at 30 °C and then analyzed by Western blotting (WB). B, HEK293T cells were transfected with plasmid vectors expressing NACA or the PP1A catalytic unit (PPP1CA). Forty-eight h after transfection, cell lysates were separated by SDS-PAGE and analyzed by Western blotting. In vitro dephosphorylation of NACA (arrow) with recombinant phosphatase enzymes (A) or in vivo co-expression of NACA (B) with PP1A resulted in a faster migrating hypo-phosphorylated form of NACA (arrowhead).