Figure 3.

PP1A mediates dephosphorylation of NACA at specific residues. A, NACA was immunopurified from HEK293T cells expressing NACA with or without the PP1A catalytic unit (PPP1CA). Posttranslational modifications were identified by LC-tandem MS. B, heat maps indicating the differential abundance of phosphorylation sites detected on NACA in the presence or absence of PPP1CA. Black indicates zero spectral counts and increasing quantities of spectral counts are represented in yellow. C, a representative mass spectra of a NACA phosphopeptide modified at serine 151.