Figure 5.

PPP1CA and BTF3 functionally cooperate with NACA to regulate cJUN transcription. A, transcriptional activity of a cJUN-responsive MMP9-promoter luciferase (MMP9-Luc) reporter construct in the presence of the indicated expression vectors. B, luciferase activity of a cJUN-responsive construct containing six tandem repeats of the AP-1 binding element (AP1-Luc) in cells transfected with the indicated expression vectors. C, effect of NACA serine/threonine-to-aspartate mutations on cJUN activity on the MMP9-Luc reporter. HEK293T cells were transiently transfected with reporter vectors together with cJUN, NACA, BTF3 and/or PPP1CA expression vectors alone or in combination and relative luciferase activity measured 24 h after transfection. Data are presented as mean ± S.D.; n ≥ 3. **, p < 0.01; ***, p < 0.001; ANOVA with post hoc test. NS, non significant.