Sedimentation velocity analysis of HO2 proteins. Sedimentation coefficient distributions for HO2(1–288) (blue trace), HO2(28–288) (gray trace), HO2(1–248) (red trace), and HO2(28–248) (black trace) are shown. All the proteins show a major peak corresponding to a monomer (2.5S and 2.6S), whereas a minor peak (4.2S), assigned as a dimer, is observed only for HO2(1–288) and HO2(28–288).