Figure 5.

RGS17 binds Mg²⁺ in solution. A, ¹H-¹⁵N 2D HSQC spectra of RGS17 alone (black) or upon addition of 15 mm MgCl₂ (red). B, structure of RGS17 where residues with CSPs greater than 0.15 ppm are shown in ball-and-stick in red. In contrast to spectra obtained in the presence of CaCl₂, MgCl₂ induces CSPs in two regions of RGS17. The locations of the Mg²⁺ ions (black spheres) are modeled based on the location of Ca²⁺ atoms observed in the RGS17–Ca²⁺ crystal structure. C, graph of CSPs for all the residues that could be assigned in the ¹H-¹⁵N 2D HSQC spectra for RGS17.