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. 2019 May 20;13(5):e0007247. doi: 10.1371/journal.pntd.0007247

Fig 3. Neutrophils in L. major-S. aureus coinfected lesions can phagocytose pathogens and have functional NADPH oxidase but are mostly apoptotic.

Fig 3

(A) Percentage of myeloid cells that are neutrophils from mice injected intradermally in the ear with phosphate buffered saline (PBS), L. major IA-2 (Lm), S. aureus Newman (Sa), or coinfected with L. major and S. aureus (L+S) for one day. (B) Numbers of polymorphonuclear cells (PMNs) positive for S. aureus LAC GFP or L. major IA-2 luc mCherry in singly infected versus coinfected lesions. (C) Dihydrorhodamine (DHR) geometric mean fluorescence intensity (GMFI) of neutrophils stimulated with PMA ex vivo in the presence of DHR to assess NADPH oxidase activity. (D) Annexin V (AnnV) and propidium iodide (PI) staining of neutrophils from PBS, S. aureus, L. major, or coinfected lesions. (E) Percentage of apoptotic (Annexin V+) versus live (AnnV-PI-) neutrophils from day 1 p.i. PBS, S. aureus, L. major, or coinfected ears. Data are represented as the mean ± SEM of two replicate experiments each with 5 mice/group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant by Student’s t test (B), or one-way (A, C) or two-way ANOVA (D) with Tukey’s post-test.